Fig 1: Lycorine inhibits U251-luc orthotopic tumor growth in vivo. a Tumor growth in the orthotopic intracranial cavity over a 40-day period was detected by bioluminescence analysis every 10 days. b Quantitative analysis of growing cells in brain bioluminescence analysis every 10 days. The means and 95% confidence intervals (error bars) are presented;***, P < 0.001, **, P < 0.01. P values were calculated using a two sided Student’s t test. p/sec/cm2/sr = photons/ second/cm2/steradian. The inhibitory effect of Lycorine on EGFR signaling pathway in U251-luc orthoropic tumor growth model were sectioned and probed with human EGF, EGFR primers (c) and anti-human p-EGFR, EGFR, PCNA antibodies (d). Human GAPDH was served as a mRNA loading control for Fig. 6c. Human β-actin was served as a protein loading control for Fig. 6d. e U251-luc orthoropic tumor sections were processed for immunohistochemical analysis to detect human GFAP, p-EGFR,Bcl-xL, cleaved-caspase 3, and Ki-67. Representative images are shown. Brown color indicates positive cells. Scale bar = 30 μm